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Abstract Objective To investigate the expression changes of microRNA- 27b in the cardiac muscle of diabetic rats and its significance. Methods After establishment of diabetic rat model, van Gieson stain was used to determine myocardial morphology and collagen volume, microRNA chips were used to screen the microRNAs associated with myocardial fibrosis, and realtime PCR was performed to validate the expression changes of microRNA. Results Van Gieson stain displayed that the collagen volume of cardiac muscle in the diabetic rats was higher than that in control group (P <0.05). MicroRNA chips uncovered abnormal expression of several microRNAs and realtime PCR validated up- regulation of microRNA- 27b expression in cardiac muscle of diabetic rats compared with control group (P<0.05). Conclusion MicroRNA- 27b may be associated with myocardial fibrosis of diabetic rats.
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