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Abstract Objective To observe the changes of NF-κB p65 and AngⅡ during various periods of myocardial ischemia-reperfusion. Methods 40 rats were randomly divided into R15min, R30min, R60min and R120min groups and control group. Rat model of myocardial ischemia-reperfusion was established based on 30-min ischemia fol owed by reperfusion for various periods (15, 30, 60, 120min). The DNA-binding activity of NF-κB p65 was detected quantitatively by ELISA. AngⅡin blood plasma and myocardium were measured by radioimmunoassay. The relevance of NF-κB p65 to AngⅡin blood plasma and myocardium was analyzed by Pearson correlation test. Results The expression of NF-κB p65 had a tendency toward increase in paral el to the prolongation of reperfusion, with the highest in group R60min, fol owed by group R120min and group R30min. There was significantly different between any two groups (P<0.05). AngⅡ in blood plasma and myocardium was significantly higher in each ischemia-reperfusion group than in control group, which gradual y increased with the prolongation of reperfusion (P<0.05). There was a positive linear correlation between NF-κB p65 and Ang II in blood plasma and myocardium(r=0.859、0.809, P<0.05). Conclusion NF-κB p65 as wel as AngII in blood plasma and myocardium increase during myocardial ischemia-reperfusion injury.
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