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Abstract Objective To investigate the expression of transient receptor potential canonical channel- 1 (TRPC1) in diabetic rat coronary smooth muscle cel s (CSMCs) and its effects on coronary vascular function. Methods Diabetic rat model was established by injection of streptozotocin intraperitoneal y. CSMCs were freshly isolated by enzyme digestion from rats in control group (n=40) and diabetic group (n=40). The protein and gene expressions of TRPC1 in CSMCs were determined by western blot and real- time fluorescent quantitative PCR technique, respectively. Cytosolic calcium concentrations before and after incubating with TRPC1 channel blocker SKF96365 were measured using fluorescence imaging of cytosolic calcium. The influence of SKF96365 on coronary function was determined by vascular tone measurement. Results The blood glucose and weight increased significantly from (7.38±0.21) mmol/L and (205.8±4.65) g before to (29.58±0.62) mmol/L and (328.3±19.57) g after diabetic model established, respectively(all P<0.05, n=40). The protein and gene expressions of TRPC1 were significantly different between normal and diabetic CSMCs (0.9802±0.0589, 0.9641 ±0.0293 vs. 1.2204 ±0.0636, 2.1484 ±0.3061, respectively) (al P<0.05). ΔRatio of calcium concentration was significantly different between normal (0.8692±0.0290) and diabetic (1.2166±0.0435) CSMCs (P<0.05).ΔRatio of calcium concentration measurements decreased significantly from 0.8439±0.0202 before to 0.0666±0.0393 after adding SKF96365 to normal CSMCs (P<0.05), and from 1.2166±0.0435 before to 0.1951±0.0277 after adding SKF96365 to diabetic CSMCs,(P<0.05). The percent dilation of normal and diabetic coronary arteries contracted by pre- treated with ET- 1, were (73.02± 5.26)%and (92.23±2.09)%, respectively, after exposed to 10μmol/L SKF96365. There was significantly different between them (P<0.05). Conclusion The expression of TRPC1 as wel as cytosolic calcium concentration increase in diabetic CMSCs with coronary artery dysfunction.
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