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Abstract Objective To investigate the protection effects of ischemic postconditioning on myocardial ischemi-a-reperfusion injury in rabbits. Methods 30 male New Zealand rabbits were randomly divided into ischemia-reperfusion (IR) group, postconditioning group, postconditioning+5-hydroxydecanoate (5-HD) group, delayed postconditioning group and sham group. The content of malondialdehyde (MDA) was measured by thiobarbituric acid colorimetry and myocardial infarct size was determined by Evans blue and tetranitroblue tetrazolium chloride and weighting method. Results The content of MDA in postconditioning group was significantly lower than that of IR and delayed postconditioning groups (al P<0.01), similar to that of postconditioning+5-HD group, but significantly higher than that of sham group(P<0.05). The my-ocardial infarct size was significantly larger in IR, postconditioing+5-HD and delayed postconditioning groups than in post-conditioning group (P<0.01). There was no significant difference among the former 3 groups. Conclusion Ischemic post-conditioning may protect rabbit myocardium from IR injury. This cardioprotective effect wil disappear when postconditioning begins 5 minutes later after onset of reperfusion, or wil be abolished by specific mitochondrial ATP-sensitive potassium channel blocker 5-HD 5.
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