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Abstract Objective To investigate the effects of valsartan on the expression and subcel ular distribution of G pro-tein- coupled receptor kinase 2(GRK2) in spontaneously hypertensive rat (SHR). Methods 18 six- month old SHRs were divided into control group (n=6), low- dose valsartan treatment group(SHRL group, n=6)and high- dose valsartan treatment group (SHRH group, n=6). Al rats feed to eight- month old and were sacrificed for heart isolation. The expression and sub-cel ular distribution of GRK2 in left ventricular myocardium were determined by immunofluorescent labeling, laser confocal microscope and western blotting. Results Compared with control group,the expression of GRK2 in total protein,cyto-plasmic protein and membrane protein of left ventricular myocardial tissue was significantly lower in SHRL group and SHRH group than in control group(P<0.05),and more significantly lower in SHRH group than in SHRL group(P<0.05). The distri-bution of GRK2 in myocardial cel membrane reduced significantly in SHRL and SHRH groups, which was more significant in SHRH group. Conclusion Valsartan reduces the expression and subcel ular distribution of GRK2 in myocardial cel membrane of SHR, which may be one of the mechanisms reversing cardiac hypertrophy.
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