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Abstract Objective To explore the effect of granulocyte colony-stimulating factor (G-CSF) on atherosclerosis ex-tracel ular matrix and tissue factor pathway inhibitor-2 (TFPI-2) in rabbits fed a high-fat diet. Methods 30 New Zealand adult male rabbits were randomly divided into control group(n=6), high-fat-fed group(n=8), normal-fed + G-CSF group (n=8) and high-fat-fed + G-CSF group (n=8) . Blood lipid level, thoracic aortic intima thickness and plaque matrix col a-gen were measured. Western blot method was used to detect TFPI-2 expression in thoracic aorta. Results The blood lipid was significantly higher in rabbits fed a high-fat diet. The thickness of thoracic aortic intima and extracel ular matrix col agen increased with fiber disarray in high-fat-fed group. These changes were significantly less pronounced and TF-PI-2 expression was up-regulated in high-fat-fed + G-CSF group. The ratios of TFPI-2 to β-actin were 1.22±0.26, 1.27±0.33, 2.45±0.22 and 1.70±0.26 in control group, normal-fed + G-CSF group, high-fat-fed + G-CS group and high-fat-fed group, respectively. Conclusion G-CSF may inhibit progression of atherosclerosis via upregulation of TF-PI-2 and reducing deposition of col agen in extracel ular matrix.
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